What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Which isomer of xylene do you use in hystology ?

Which isomer of xylene do you use for the preparation of histological tissue sections - meta para or ortho?

02 February 2021 833 4 View

Can we re-freezing of brain tissue homogenates?

Hello! We use homogenates of mouse brains for our aims (determination of cytokines by the ELISA method). After removing the brain, we freeze the tissue in liquid nitrogen, then store it for a...

19 January 2021 5,203 1 View

What is a functional blocking assay for an antibody fraction?

I will be grateful if you could explain me or give a reference where I can read about: - What steps a functional blocking assay could include? - Are there other possibilities to prove presence...

17 November 2020 3,032 3 View

Should antibodies against the receptor binding domain of a spike protein bind to a complete spike protein?

The receptor binding domain of the coronavirus spike protein has been expressed in Escherichia coli. Antibodies were obtained to the purified protein. These antibodies do not recognize the spike...

13 October 2020 327 5 View

How to perform overlay from different experiments in FACS Diva?

Dear colleagues! I am in desperate need to do overlay data collected on different days in FACS Diva (need to compare some markers expression levels on different days of cell growth). The settings...

28 July 2020 1,779 3 View

How to correctly choose population strata and interpret results for the detection of loci under selection from RADseq data using Bayescan?

I am working on the analysis of fungal populations sampled from 12 geographic locations using RADseq data. The dataset contains 7439 polymorphic loci with ~10 snps per RAD locus (75,482 snps...

19 May 2020 643 3 View

Good entry literature on philosophy and/ or ethics of technology?

Hello, I am searching for a good introduction or overview on the topics, methods and research of philosophy or ethics of technology (separately). Any suggestions? Thanks in advance! Hallo, suche...

23 April 2020 5,096 1 View

What will be the real consequences of the COVID-19 pandemic on corporate credit rating?

We know that with Basel all banks adopt a rating system to evaluate their loans and to calculate their regulatory capital. companies have always had problems with the rating calculated by banks...

08 April 2020 9,034 7 View

Editing experimentsl and control cells with the lentivirus, I have prevailing signals from cell immune response on virus. How can that be?

Hello everybody! We have modified cells with the lentiviral construction and then analyzed the result by RNAseq. I've made gene set enrichment analysis in R-package fgsea. And obtained the result,...

03 March 2020 9,876 1 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

Has anyone done Archard Wear Simulation of GFRP Composite Plate in ANSYS with ACT extension or UDF?

I'm looking to do a sliding wear (plate-on-plate) simulation of GFRP composite plates with Archard Wear Model. Any hints or example steps are appreciated.

17 February 2021 1,447 2 View

How to visualize stress in a coating defined by "Skin" in Abaqus?

Hello All! I'm trying to model a contact between a coated substrate and a tip in Abaqus. The coating is ultra-thin compared to the geometry of the substrate. So I defined the coating using the...

16 February 2021 1,594 3 View