Did anybody worked with EphA family protein expression? MW 130kDa . I am not getting any expression. Do you use different lysis buffer or RIPA?

I am working on EphA2, pEpA3 expression with different cell types (MD-MBA231, MOLT4) for the expression of EphA family proteins, They are of high molecular weight 130 kDa. I use RIPA buffer and...

01 December 2020 8,610 1 View

What statistical test should I use if I want to compare values of temperature taken at aural, forehead and inner wrist ?

I basically want to see if there is a difference between these sites. Especially between the forehead and inner wrist as many patients are not very happy with temperature reading taken at the...

05 October 2020 3,151 12 View

Need weather and metrological data for forecasting or predicting the agriculture in Saudi Arabia?

Need weather and metrological data for forecasting or predicting agriculture in Saudi Arabia? This is for the purpose of one of the project by my fellow researchers. Kindly share the same.

11 August 2020 8,163 4 View

What are the high impact journals available in sustainability and sustainable development domain?

What are the high impact journals available in sustainability and sustainable development domain?

24 June 2020 3,979 11 View

What are the COVID-19 papers retracted from journals?is it clinical, treatment, diagnosis or AI based prediction? Why?

What are the COVID-19 papers retracted from journals? is it related to clinical, treatment, diagnosis or AI-based prediction? Why

07 June 2020 7,301 3 View

Any suggestions for SCOPUS Indexed journal for social science field, specifically for education?

I'm looking for any SCOPUS indexed journal to send my article. Social science specifically in education field with minimum waiting period for the acceptance. I did contacted some journal editors...

20 April 2020 8,242 1 View

Hydroxychlorine was proposed as drug for corona viral disease (COVID-19). Is it good for treatment?

It might induce immunosuppression.

03 April 2020 3,740 4 View

Quantitative & Qualitative perceptive. (mix mode)?

Dear All, My research topic is socio-economic impact of employment of Filipinos Maids in Singapore. My research will be both Quantitative & Qualitative perceptive. (mix mode) My question:...

19 October 2019 5,959 8 View

How can we determine the sample size from an unknown population?

Please provide example too, im new ...

09 April 2019 5,724 4 View

Transformed colonies with GFP showed Faded Fluorescence ?

Transformed Yeast colonies with a Plasmid having GFP, showed faded Fluorescence. It was light yellowish in colour rather than the usual Green Colour . What could be the reason ? The Microscope was...

28 December 2018 7,516 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

No title

01 March 2021 3,905 5 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

What does it require to be ready for e-Commerce in developing countries?

Most developing countries are not ready for e-Commerce? What dimensions and indicators are there to measure e-commerce readiness of a nation? Please share framework or model and evaluation...

22 February 2021 8,132 6 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

How to disaggregate country data into city ones?

I'm using a number of indicators in my research which are not available at a sub-national level. Is there a reliable method for disaggregating the country data into city ones? (preferably with a...

16 February 2021 6,861 3 View