Among all the environmental pollutants globally how much % out of 100% is due to chemists?

Environmental pollutants and hazardous are major issues through which the modern world is suffering. Among all the environmental pollutants and hazardous materials how much contribution is from...

31 December 2020 6,039 3 View

Among All the environmental pollutants how much % out of 100% are due to chemists?

Environmental pollutants and hazardous are major issues through which the modern world. Among all the environmental pollutants and hazardous materials how much contribution is from chemists (out...

31 December 2020 833 7 View

How to prepare selenium nanoparticles from selenium dioxide?

For the preparation of selenium nanoparticles mostly selenious acid is used however if someone try to prepare it from selenium dioxide what will be the proposed procedure or mechanism?? Is it...

07 December 2020 4,979 4 View

What are the exact phytochemicals present in the plant extracts which act as reducing and stabilizing agents in the green synthesis of Nanoparticles?

There are hundreds of research articles published on the green synthesis of nanoparticles of various metals and metal oxides nanoparticles using plant extract (where mostly the extract of leaves...

30 November 2020 735 6 View

How can I derive the Fresnel reflection coefficient in matrix form?

I need help in deriving the Fresnel reflection coefficient in matrix form for the 2 X 2 reflection matrices. After diligently searching online, I noticed I could get the steps in a book called...

11 October 2020 215 3 View

How to collect data for Regional Research Study?

08 October 2020 8,003 4 View

Can we test the same hypothesis by using two different techniques Regression analysis (SEM) and Variance analysis (ANOVA)?

I have model where I have four dependent variables in nomological order and 3 independent continuous variables . To test casual relationships I first compared means of outcome variables across...

19 June 2020 7,764 8 View

Is there an Instrument to Assess Faculty Members Readiness to Implement Knowledge Management?

I am searching about an Instrument to evaluate readiness of faculty members to implement knowledge management?

01 March 2020 5,394 3 View

Could you please help me by solving the following integration?

Dear Colleagues, I'd be grateful if you provide me the solution or give an idea to solve integration. Thanks in advance. Huda

12 February 2020 1,989 15 View

What is the best way to approach a scientist for Post Doctoral research position?

I have completed my Ph.D. During Ph.D., I had worked on various aspects of the pathogenesis of Candida glabrata. However, in Post Doctoral studies, I want to work on immunological aspects for the...

11 February 2020 4,482 8 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How can I quantify the color of a solution?

I know colorimetry has been the standard way by way of measuring the concentration of a particular compound (solute) in a colored solution (solvent or measure quantities of a particular compound...

08 February 2021 2,673 1 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View