In some research paper 24 , 30 insect per treatment had taken for study. Is this possible to take 10 insect per treatment and carry out experiment in triplicate. Please guide...
you can run probit analysis with 10 insect per treatment which will be triplicate. that means the total insect used per treatment will be 30. What I want to suggest you is that, when you increase sample number, you are approaching to the actual reality in the population. statistically, sample number below 30 is not recommendable. so to summarize your question, probit analysis is possible with your sample (10), but increasing sample will be good for better interpretation and acceptance of your result.
I am following this discussion. I have run probit analysis using spss in which LC50, LC90 were displayed but no confidence limits. I don't know what happened , please I need your assistance. Thank you