Dear colleagues, we are going to introduce HLA typing based on Luminex technology. Does anybody can help in this ?

More Giorgi (George) Babuadze's questions See All

What should Berlin do as a city to become as impactful as London and Paris in World Football?

Please go through my Abstract. I can also share a proposed Thesis Outline.

04 August 2024 2,077 0 View

How can we separate diastereomers of larger organic moiety?

For example, oxazine type moieties which forms diastereomers, how the separation can be done? Chiral HPLC is the only possibility?

10 July 2024 1,519 3 View

How does one remove a withdrawn article from a journal's plagiarism test repository?

We submitted an article to a journal, which subjected it to a plagiarism test. Afterward, we withdrew the paper and submitted it to a different journal, only to discover it now has a very high...

07 June 2024 4,688 3 View

Is it possible to use commercially available 3% or 6% hydrogen peroxide for the hydrogen peroxide scavenging assay?

Good day! I am currently testing for the antioxidant activity of my extract via the hydrogen peroxide scavenging assay. I have already done the experiment in six trials using 30% hydrogen...

27 May 2024 4,677 3 View

Where did the 'respond to request' button go?

The format to requests has changed. I simply want to respond to a request and can no longer find any way to do this. The 'help' section regarding this is 2 months old. Thanks, George

30 April 2024 250 0 View

Can migrants apply for economic asylum in your country as part of his recognised HUMAN rights?

The harsh reality facing the world and migrants majorly is the wide economic disparity and Inequality fuelled by some rich world economies exploring resources and failure to meet social...

09 April 2024 1,420 0 View

Is it possible to validate the studies like Implantation, Acute toxicity, skin sensitization, irritation on animals ?

Is there any method validation procedure for toxicological studies like Implantation, Acute toxicity, skin sensitization, irritation on animals. Generally these studies will conduct on animals.

28 March 2024 280 0 View

How do I fix significant Quality control Drift with the GFAA?

I run an Agilent Technologies Graphite Furnace and a recurring problem when analyzing Lead is that the continuous calibration verification, (CCV), QC slowly drifts lower from the target (20ug/L)....

27 March 2024 3,609 0 View

In traditional taxonomy, can a species image be prepared based on only one half and mirrored the other half in photoshop?

Recently, people working in faunal research have practice to photomontage when identifying a species (in most cases they are not professional taxon experts), and they make a mirror image of one...

21 March 2024 6,684 8 View

Hi, can ethanol show fluorescence?

Since my compounds are soluble in ethanol, pure ethanol is showing a broad peak at 371 nm. Is it possible?

20 March 2024 4,843 4 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

Assistance with MTT Assay Data ?

I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...

26 July 2024 7,511 3 View

Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View