Does re-stimulation of T cells with PMA and Ionomycine before staining for intracellular cytokines for flow cytometry affect reliability of the assay?

I understand that to be able to measure intracellular cytokines there is a need to restimulate with PMA and Ionomycin but I don't understand how the results will be reliable after that. PMA and...

18 January 2021 7,240 7 View

In meta-analysis of binary outcomes, what should we do when the occurrence of outcome is zero in both groups?

In conducting meta-analysis of binary outcomes (in RCTs), in case the risk ratio has not been reported, what should we do with the studies that the number of outcomes occurrence is zero in both...

09 January 2021 2,802 5 View

How to calculate ratio of means and ratio of geometric means?

In conducting a meta-analysis, sometimes, some studies have reported geometric mean, while others have reported arithmetic mean. One solution for pooling these studies is calculating the ratio of...

11 December 2020 4,663 3 View

How To Find Electrical Nanodipole and Magnetic Loop Dimensions At Optical Wavelength?

I would be grateful if you could suggest a paper or book which provides an equation to determine the dimensions (length) of an electrical nanodipole and magnetic loop antenna at desired optical...

17 October 2020 2,421 4 View

What effect do epidemics have on cerebral physiology?

How trauma and fear of infection can affect brain functions and structures?

10 October 2020 6,642 1 View

Can anyone advise me in selecting a good pair of universal primer/primers for identification of lactic acid bacteria (probiotics)?

when I blast the existed primers specially 16s rDNAs , I cant get good results.

21 September 2020 1,584 4 View

Could anyone help me searching for protocols in fiber analyses ?

Hello, I am looking for protcoles to evaluate the following components: TDF : total dietary fiber, SDF : soluble dietary fiber, IDF : insoluble dietary fiber et TDN : total digestive nutrients.

06 September 2020 371 2 View

Any journal which has no publication fess for pops removal or water treatment through adsorption?

I am looking for a reputable journal for publication of research article of MS thesis. Kindly suggest me some journals related to water treatment through adsorption which is free of cost.

13 July 2020 8,259 6 View

How much water we should add to paint if we use a based water binder (resin) or there is no need for adding water with such binder?

I want to know if the water included in resin is enough as a solvent or that water is not counted and I should add water as solvent to paint?

21 June 2020 322 6 View

In your opinion, do I choose a certain disorder to study its relationship with culture, or better not to define the disorder?

Dear colleagues Who can help me find studies or references that study the relationship of culture to mental disorders. I would be very grateful for your help. :)

26 April 2020 3,003 36 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View