Example : I have the following sequence, of the penetratin peptide, conjugate with Cf:
RQIKIWFQNRRKWKKNH2.
The synthesis is done m=150mg resin, the resin substitution 0.65mmol/g.
MW=2471.301g/ mol.
I have got as crude after the synthesis m=134 mg. and I purify 20mg, I have got 4.88 mg.
Thank you
The crude material contains salts and byproducts (TFA salts, ...).
Calculate the theoretical yield based on resin capacity (0.15g* 0.65mmol/g), peptide molecular weight (add reasonable counter-ions, if necessary).
Then express real yield as a percentage of the theoretical yield. Done.
(if you purify only a fraction of the total material, adjust accordingly)
Calculate the yield of the peptide by comparing the amount of purified peptide obtained to the initial amount of starting material. The yield is usually expressed as a percentage and can be calculated using the formula:
Yield (%) = (Mass of purified peptide / Mass of starting material) x 100.
Desh Deepak A P Singh Chauhan How would you take account of all the removed protection groups (Fmoc, Boc, MTT, etc)?
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