Is methylene blue staining necessary for Macroscopic evaluation of the incidence of polyps in a DMH induced colon cancer in mice?

Am working on a DMH induced colon cancer in mice and i would like to know, could polyps or ACFs be counted through visual macroscopic examination without methylene blue staining ? Thank you.

01 February 2021 4,237 2 View

DFT- B3LYP calculations of functionalized cellulose monomer ?

Has anyone already performed DFT calculations for cellulose and functionalized cellulose monomers? How can we do it to calculate the bond dissociation enthalpies and IR frequencies?

23 November 2019 2,665 3 View

What are the best strategies for improving the quality of life of children with learning difficulties?

quality of life

23 May 2019 940 22 View

What molecules or hormones can I target with immunohistochemistry in the study of the impact of heavy metals on fertility?

please, what molecules or hormones can I target with immunohistochemistry in the study of the impact of heavy metals on fertility? and what antibody can I use? And thank you in advance

16 January 2019 8,972 3 View

Could you suggest me a solvent to solubilize Fomlin ?

Could you suggest me a solvent to solubilize Fomlin ?

16 November 2017 2,925 1 View

Searching timers like Trickle ?

One of the key design principles of RPL protocol is minimizing the routing control overhead and signaling data as a mechanism to reduce energy consumption and even enhance the reliability of the...

23 May 2016 1,668 2 View

How can I compute divergence between two vocabularies using Kullback-Leibler distance?

Having two vocabularies, how can I compare them using the Kullback-Leibler distance?

24 November 2014 1,099 3 View

Does anyone have some advice to improve the in situ hybridization to investigate TIMp-2 in HPV-16 infected oral squamous cell carcinoma?

I have a problem in the nuclear staining of the cells.

01 May 2014 7,419 1 View

What are the mechanisms that affect the replicative senescence of primary somatic cell culture?

Is a shortening of the telomeres one of the limitations?

25 April 2014 1,365 1 View

Teaching by e-learning or face to face in higher education ?

Dear colleagues, Due to Covid 19, we got used to do teaching via e-learning. do you prefer to continue with e-learning or do you miss face to face ?? what is the impact of this change on the...

01 January 1970 3,183 26 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

Does anyone know of a predictable relationship beween surface gravity and atmospheric composition?

The attached file demonstrates that there is a predictable relationship between the structural density of a terrestrial body and its none-rotating surface gravity. Using Newton's gravity law of...

26 February 2021 2,317 3 View