Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Ideas for Discussion of a Case-study analysis?

I am currently researching the demotivating factors and remotivating factors of high school students in my country. My study only has 13 participants, and I groups them into 3 groups depending on...

02 December 2020 2,288 3 View

About cross-case analysis?

Currently, I have finished interviewing my high school students to learn about their demotivating and remotivating factors. I'm trying to use case-study method to analysis data. In my...

17 November 2020 7,989 2 View

About researching EFL learning demotivation and Straussian grounded theory?

Currently, I am conducting a MA dissertation by using Strauss and Corbin's Grounded Theory. My topic is researching EFL learning demotivation of high school students (16 to 18 years old). My main...

13 July 2020 6,773 4 View

What kind of optimization is done through genetic algorithm in architecture?

Difficulties and types of optimization

11 May 2020 6,177 1 View

Which Grounded Theory should I use?

I am a novice researcher who is writing my dissertation. My topic is high school students' demotivation and their coping strategies in my country. Currently, I am thinking about using Straussian...

15 February 2020 2,243 3 View

Command line for results in autodock4?

Dear professors, Could you please tell me the command line to transfer dlg results file into pdbqt files in COMMAND PROMT? Im very hopeful for your advises. Kind regards.

18 May 2019 4,205 2 View

Add charges to Zn in protein?

I am now using Autodock Tools and Autodock 4 to figure out the interaction of ligand-protein. However, there is a problem I have to face now is that the Zn in the protein has 0 charge instead of...

15 May 2019 4,171 5 View

Questions about extraction process in an article?

Hi, I am going to use the extraction procedure from an article, however, I don't understand the bolded step. Could you please explain how I precipitate the protein in cold acetone and what the 5:1...

19 November 2018 8,449 7 View

Band at double molecular weight?

So I was analyzing HSP70 in plants by Western blot. However the bands I got was at around 150 kDa instead of 78 kDa indicated by the product information. I realized I didn't heat the...

28 October 2018 4,500 4 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

No title

25 February 2021 5,713 1 View