Hi, I have a problem with my mouse Adipose MSC primary culture?

I am working on mouse adipose mesanchymal stem cells. After removing the mouse peritoneal fat and extracting, I do the primary culture, I used DMEM F12 medium with 15% FBS, but with this, the...

13 February 2021 9,838 1 View

How to estimate total electron content using GPS data (with MATLAB)?

06 January 2021 1,110 2 View

Do you think that our world will reach 100% of its electricity needs from clean energy sources?

Do you think that our world will reach 100% of its electricity needs from clean energy sources?

02 January 2021 3,059 62 View

If you have solar PV panels and a grid-tied inverter, what happens if the system is connected to the grid without a net meter?

If you have solar PV panels and a grid-tied inverter, what happens if the system is connected to the grid without a net meter?

13 December 2020 8,874 3 View

What are the main differences between Photo Voltaic (PV) solar sources and Solar Water Heaters (SWH), especially from economical aspect?

Is it feasible to replace my Photo Voltaic (PV) solar panels system by Solar Water Heater (SWH)?

02 December 2020 4,740 7 View

How can I find the area of contour with magnitude less than specific number?

Hello everyone I have results from fluent and I want to determine area with magnitude less than specific number for example for velocity or TKE. and also how can I determine Max and min diameter...

02 November 2020 5,863 3 View

Why residuals stay at the same range in vof model in Fluent and it doesn't converge?

hi I'm modeling a 3d channel with vof , k-epsilon RNG with non-equilibrium wall functions , for bc I have velocity inlet, pressure outlet and for top of the domain I have used pressure outlet...

21 August 2020 5,926 7 View

How I can obtain the cartesian coordinates of a complex without single crystals for gaussian calculations?

When the single crystals of complex are not formed, how we can obtain cartesian coordinates for Gaussian calculations? We want to predict kind of interaction or coordination of manganese to amino...

18 August 2020 8,146 3 View

I am confused about the editor's request (I had submitted a manuscript and then major revision was requested). Can anybody help me?

I had submitted a manuscript to a journal. After a while, they requested major revisions. The end of their request, they mentioned that: "You will be unable to make your revisions on the...

09 August 2020 5,436 7 View

Is it possible to extract RNA and protein from 96-well plates?

I am doing a knockdown test in 96 well plates and then I need to extract RNA and protein from my cells. I'm not sure that the number of cells in 96 well plates is enough to these extractions....

12 June 2019 5,459 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View