Are there any corporations that have launched Enterprise MOOCs to the public?

I am studying Enterprise MOOCs and can't seem to locate very many empirical articles for this area. The model I am interested in is similar to openSAP (https://open.sap.com). Please share any...

28 September 2020 6,813 2 View

HEK 293 MSR cells die/detach after transfection?

We have used TransIT-X2 (Mirus) for years to transfect different cell lines, but suddenly I am having a lot of trouble with my HEK 293 MSR cells becoming very round and dying/detaching soon after...

07 February 2020 6,375 3 View

Does anyone have a good reference for gestational sizes of Sprague Dawley rats?

Looking for data on gestational sizes of SD rats and also if an atlas of development exists. Thank you!

12 April 2018 6,819 1 View

Any insights on using USGS water quality data (ie total dissolved solids) in a statistical analysis when samples are dried at different temperatures?

11 December 2016 3,264 8 View

How do you include a theoretical framework in a research proposal for an exploratory study on knowledge and beliefs?

I am a Honours student, applying for the masters programme in counselling psychology. I am expected to produce a research proposal for my possible master's thesis. I have decided on doing an...

20 August 2016 9,989 11 View

Does anyone have knowledge pertaining to expressing bacterial proteins in mammalian cell culture? Do you need to worry about codon usage?

We have created a modified toxin plasmid using a mammalian receptor domain. We are trying to express the modified toxin plasmid in cell culture, and isolate the protein. We need mammalian cell...

08 June 2015 186 3 View

What brands of Ni-NTA resin work best?

I am doing a gravity purification of 6x-His tagged staph nuclease in a pET28b+vector under native conditions, with no special conditions. This protein is very easy to purify and usually boatloads...

19 May 2014 2,517 10 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The Role of Town Planners in CAV Use?

Colleagues and I are about to begin a project investigating the role of town planners and local authorities in establishing the introduction of connected and autonomous vehicles (CAVs). What...

21 February 2021 2,247 3 View