Can you suggest a good domain identification tool?

I am working on a project to identify sensitive information from text and I am looking into the below categories: -adult content -health -politics -crime Can someone suggest an open source tool...

16 February 2021 4,844 4 View

I am trying to run a simulation on LS-Dyna and I am facing the error below with Nodal rigid constraints. Can anyone tell how to fix it?

*** Error 20393 (STR+393) Nodal rigid body ID 85000010 contains node ID 83500180 already used in a rigid body/nodal rigid body definition.

20 January 2021 2,019 1 View

How the microplastics levels in freshwater and marine water ecosystem be controlled?

Microplastics have been found in land, air, freshwater, effluent from a wastewater treatment plant and even in tap water and bottled water. The small plastic particles (less than 5 mm) pose a...

19 January 2021 8,637 3 View

What to do when RT PCR becomes positive again after 15-20 days of 1st sample positivity?

We are getting some patients who were initially positive of RT PCR for Covid. But in retesting after 15-20 days or even 20 days after those patients remain still positive. Is it for dead RNA...

17 August 2020 9,552 9 View

Can you please recommend a good open source text classification tool (NLP)?

I am working on a social media dataset and want to classify if a public post leaks sensitive information or not. The text includes slangs and emoticons too. Can you suggest an open source NLP...

30 June 2020 1,678 7 View

How to calculate the HCACF (heat current autocorrelation function) in LAMMPS?

I have obtained the J0Jt.dat file by Molecular Dynamics using the LAMMPS software. How can I obtain the curve for HCACF (heat current autocorrelation function)?

10 June 2020 6,024 1 View

Kindly suggest the best Labelling tool/ Python script to create labels on images(very big in size 5000 * 8000 pixels)) ?

Hi everyone, I want to create labels on images, please suggest me the best labelling tool for the same, any python script would be preferred and image dimensions are very big as mentioned...

16 January 2020 1,604 3 View

What is the best tool/way for fast parsing of ~80 GB pcap.gz files?

Any suggestions on how to parse such large pcap.gz files of telescope data from caida. If I use tcpdump or tshark, it is taking too long to parse them. Also, I have a big list of filter...

03 December 2019 7,750 7 View

Where can I find a complete list of Cloud Providers of the world?

I could find this: https://en.wikipedia.org/w/index.php?title=Category:Cloud_computing_providers&pageuntil=Proofpoint+Systems%2C+Inc.#mw-pages Does anyone know of a more comprehensive...

13 October 2019 614 6 View

IRS mission Thermal Imaging?

Hello, Anyone aware of any Indian Remote sensing Satellite having Thermal sensor (for UHI effect)? Data is free or paid?

24 September 2019 2,062 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View