Hello Amin Saiff Johari

Saos-2 cells cannot be cultured at 34°C. Most mammalian cell lines, including Saos-2, have a specific optimal growth temperature, that is 37°C. Maintaining cells at this physiological temperature ensures proper metabolic processes and cell function. Culturing at temperatures lower than 37°C, such as 34°C, is not typical and may negatively impact cell viability, proliferation, and differentiation.

Human fetal osteoblast cell line, is cultured at 34°C for their optimal and accelerated proliferation as an immature, undifferentiated state, while a higher temperature, like 37°C, is used to induce differentiation and a mature, osteoblast phenotype. This two-temperature method allows for studying both the proliferative and differentiated functions of the cells.

Best,

I have now switched the culture to be at 37 degree. While im 34 degree the cells are doing just fine. However I am thinking of switching to another medium to observe its proliferation rate. I thought of making a standard curve for cell density vs incubation time in different media.