22 May 2025 0 4K Report

Precauciones para identificar rápidamente y a tiempo este síndrome.

More Allison Daianna Luján Lizárraga's questions See All
How to do a systematic review?

Hello, I am working on a systematic review. I have my topic, and I am searching Scopus and Web of Science, but I want to search Google Scholar to compare the results. Does anyone have any advice...

10 June 2024 9,602 0 View

Brain cryosections cracking after permeabilization and drying?

I am doing immunofluorescence of mouse brain cryosections. I collect 25 um sections onto 1% gelatin coated coverslips, let them dry ~30 min, then peel off the OCT, rehdyrate the sections with 0.1...

14 December 2023 4,054 0 View

How to obtain empty vector from plasmid?

I require the empty vector of this plasmid: https://www.addgene.org/40346/ The ends have been destroyed: 5′ cloning site XhoI/SalI (destroyed during cloning) 3′ cloning site SalI/XhoI...

12 December 2023 1,220 4 View

How to calculate MFI comparing bimodal and normal distributed curves?

Dear researchers, A surface marker presents as a bimodal peak, however upon treatment the marker is a singular, wider spread peak. How would I calculate a comparable MFI between the two...

03 December 2023 7,404 0 View

Could you explain why there is a difference in gene expression between a RT-PCR and a no-RT control PCR when the same genes are analyzed?

In particular, can you explain why a gene is more highly expressed in PCR without reverse transcriptase than in RT-PCR? I have performed a PCR with reverse transcription (RT-PCR) and a negative...

23 November 2023 9,986 1 View

Do you work in higher education as a professor or lecturer?

If so, I invite you to share your opinions in a quick seven-question survey I put together for my grad school course in applied user research for UX. We want to identify which features are the...

21 October 2023 2,401 7 View

Brief measure of self-determination at work (autonomy, competence, relatedness) for use with bachelor's level helping professionals?

I am seeking a brief measure of self-determination at work (autonomy, competence, relatedness) for use with bachelor's level helping professionals. The study will consider whether specific...

17 October 2023 6,525 0 View

Are there alternatives to Neurolucida to trace stain from confocal images stacks?

We are seeking to count cell death stain from confocal image stacks; however, Neurolucida is quit expensive. Are there any alternatives that you recommend? Is ImageJ viable?

08 October 2023 1,263 1 View

Prolific vs MTurk? Alternatives?

I wanted to get feedback from people who have used Prolific and MTurk (or other services) for participant recruitment.

02 July 2023 7,506 0 View

Double staining with same host?

Hello, I am trying to double-stain with Pax 6 and Cleaved Caspase 3. I have looked into the markers but unfortunately, both antibodies are produced in rabbits. I tried Pax 6 and Conjugated Cleaved...

27 November 2022 3,145 2 View

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How Can I Resolve a Persistent Unwanted NMR Peak at 1.25 ppm?

Hello everyone, I am facing a consistent issue in my NMR spectra with an unwanted peak appearing at 1.25 ppm. This peak seems to vary with the amount of sample: it becomes more pronounced with...

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What are the criteria that must be retained for the development and validation of a qualitative NMR method ?

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Quelles perceptions et quelle place pour la langue chinoise en Afrique ?

Lorsque nous nous posons cette question , nous nous basons sur un fait et constat très simple : Dans les manuels d’apprentissage du chinois, qui tiennent lieu de programme pour l’instant dans les...

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