Theoretical computer virology ? is still alive ?

HI Everyone! I am asking to know by you, expert in cybersecurity and mathematics, if Computer virology (the one of Cohen and Andler) is still an active field of research. That research made in...

08 February 2021 7,613 6 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

In opensees is the ReinforcingSteel material sensitive to units of measure?

20 January 2021 7,043 2 View

Why green band returns more inliers than NIR band during image registration?

Hello I'm using image registration over the bands of the micasense red-edge camera. Using RedEdge band as the fixed image. After removing inliers I can notice that inliers of the NIR band are...

13 January 2021 3,980 2 View

How do I organize items to repeated surveys for quantitative and qualitative analysis?

Hello fellow researchers! Newbie here :) I created a survey with open-ended and Likert-type questions. The survey was administered nine times to the same people using google forms. I plan to...

01 January 2021 3,921 3 View

Is there a better library or function for plotting CFA with many observed variables in R?

Hello! I'm kind of new in using R for doing EFA, CFA, and SEM. I just followed a guide to perform CFA on my data to see how my context fits the ITC-SOPI model in a VR photo-based tour. I used...

23 December 2020 1,679 2 View

Problem executing MrBayes. Help?

Hi, I'm trying to run MrBayes 3.2.7 with a matrix of 37 species and 20561 nucleotides. I consistently receive the following error: MrBayes > Execute...

18 November 2020 3,867 3 View

Which commercial DNAse do you recommend me?

Which is the best DNAse (or DNAse kit) you have worked with? I am looking for a good DNAse that can remove DNA from an RNA sample in one pass. Material would be used for future RT-qPCR analysis....

18 November 2020 8,769 3 View

Is there same kind of limit when annealing two DNA products?

Hi there I'm planning on annealing two PCR products to perform some mutagenesis in a plasmid. I was recommended to try and keep it to 600pb each, for example, but for one of the plasmids there...

15 November 2020 8,656 3 View

What is the relationship between plant hormones and plant allometry?

Hi, i'm looking for bibliographic references that explains the relationship between plant hormones and plant allometry (relation between the size of an organism and aspects of its physiology and...

21 September 2020 9,653 6 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

How much should I centrifuge cells in agarose-coated plates to get spheroids?

Right now I am using HT29 cells, but I will also use HCT116 and probably breast cancer cell lines.

09 February 2021 2,063 6 View

C-4 I cervical cancer cell line tissue culture protocol?

Hi there! My lab has been brought an array of C-4 I cell line. The cells did not succeed in attaching to the bottom of the flask and remained floating in the media. Although we followed the...

04 February 2021 10,018 5 View