30 May 2025 1 4K Report

PARVOVIRUS CANINO

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Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,198 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 477 5 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,162 0 View

Similar questions and discussions
I have faced a problem to amplify VP2 gene of porcine parvovirus 1,2,3 and 5? Please suggest me how can i solve this problem?

Till now i haven't got any PCR amplified vp2 gene bands on specific annealing temperature and in step up PCR.

08 February 2021 1,170 4 View

Is there a comprehensive database for Virus Real-Time Primers and Probes ?

Dear All I am looking for a database for tested Real Time PCR primers and probe sequences for the following viruses for diagnostic purposes. Does the USAID PREDICT project or the Global Virome...

01 February 2021 9,210 1 View

What is the relation between bootstrap and homoplasy?

I am working with the VP2 protein DNA of carnivore parvoviruses and sequences are only of 1300bp. I obtained the following phylogenetic tree (Maximum likelihood, 1000 replicates) and the reviewer...

14 February 2019 8,713 0 View

Porcine vascular endothelial cell isolation - from the aorta?

Hello, Are there any protocols for the isolation and purification of porcine vascular endothelial cells (from the aorta)? I would like to have my study from the primary culture instead of...

09 October 2017 9,600 3 View

Hi, I'm unable to sequence a DNA fragment with a polyA region ~13bp. Any advice on if additional Mg2+ may help & suggestions for a gradient protocol?

I am sequencing overlapping regions of the VP2 gene of Parvovirus. We get a good reverse sequence but chromatograms following the polyA region in the forward sequence have very low and mixed...

23 July 2016 9,307 15 View

Hi, I'm unable to sequence a DNA fragment with a polyA region ~13bp. Any advice on if additional Mg2+ may help & suggestions for a gradient protocol?

I am sequencing overlapping regions of the VP2 gene of Parvovirus. We get a good reverse sequence but chromatograms following the polyA region in the forward sequence have very low and mixed...

06 July 2016 8,595 16 View

Transmission of HSV by allogeneic serum eye drops - is it possible?

Hello! We produce allogeneic serum eye drops from (SED) whole blood donation and test them for HIV-1/2, HCV, HBV, HEV, Parvovirus B19, Syphilis, CMV and HSV. We look for antibodies and for the...

28 June 2016 3,992 0 View

How could I isolate a specific virus from a multivalent vaccine?

I want to isolate canine distemper virus in cell culture (Vero) from a multivalent vaccine that also has adenovirus, parvovirus, parainfluenza (all attenuated) and Leptospira (inactivated). Is...

15 June 2015 573 3 View

What is the best protocol for PCR purification of Canine Parvovirus extracted from spleen of small mammals?

I have used dreamtaq and amplitaq with both plain and nested PCR but it does not seem to work really well, The image is really smeared with a lot of non specific binding when extracted on 1.5%...

13 May 2015 2,709 3 View

Do you know if duodenal papillitis could be an unusual complication of Human Parvovirus B19 Infection?

I recently observed a young immunocompetent girl with obstructive jaundice related to  duodenal papillitis and detection of Parvovirus B 19 IgM

12 April 2015 5,205 1 View

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