I have extracted plant material first through petroleum ether then Chloroform then Ethyle acetate finaly with methanol in the methanolic extract crystal are form what it indicate what could we the compounds?
It may be pure compound/complex mixtures, should go first for TLC then LC-MS
Hello Kesar,
many thanks for sharing this very interesting technical question with the RG community. Of course it depends on the individual plant and its ingredients if a single component can be isolated in crystalline form after extraction. Thus in your case it is difficult to provide a qualified answer without knowing which plant material you are working with. In this context I just came across a very interesting study in which the isolation of pure, crystalline rutin from alcoholic extracts of Sophora japonica L. has been reported. Rutin is a flavonoid derivative which has been found in more than 70 plants growing e.g. in China and Vietnam. Here is the article:
Crystallization-Based Isolation of Pure Rutin from Herbal Extract of Sophora japonica L.
Article Crystallization-Based Isolation of Pure Rutin from Herbal Ex...
The paper has not yet been posted as public full text on RG, but four of the authors (who are all based at my university) have RG profiles. Thus you can easily request the full text of this article directly from one of the authors via RG.
You might also want to have a look at the answers given to the following closely related question which has been posted a few years ago on RG:
Crystals in plant extracts?
https://www.researchgate.net/post/Crystals_in_plant_extracts2
(7 answers)
Good luck with your work and best wishes, Frank Edelmann
As mentioned by Suresh Kumar Dev earlier, you may try TLC. This is likely to indicate whether your crystals are from a pure compound or not.
Melting point (MP) determination may also provide with useful information. A sharp MP would indicate that your crystal is from a single pure compound.
Slow rate of heating and a keen, observation is the pre-requisite.
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