The simplest method would be to turn the cryostat off and let it thaw overnight. You'll need to remove as much of the meltwater as possible before cooling the machine back down and give it at least 5 hours to reach a stable temperature before use.

There might be a problem with the thermostat or cooling elements that caused this issue since there doesn't seem to be any other areas that have frozen over. If the head freezes over again after thawing, the machine will need to be serviced.

I have never seen this kind of situation, I have used some of these from different companies. Shut down for overnight. Also you can call Leica Technical advisor. Leica sale agent in your area can have a first look and than technical advisor will advise you to fix problem permanently. good luck

Hello,

the remarks of John and Subash will be very helpfull for you (I recommended them yesterday)!

However, after one night of sleep I had another possible idea:

Do you (or your Labmates) allways close the freezer chamber, when you are not working with the croystat? If someone forget to close it over night, then things like shown on your picture can occur.

What kind of temperatures do you use for cryosectioning?

In our Lab (in Germany, not moisty and about 18°-21° C room-temperature) the ideal temperatures for cyrosectioning were: Chamber -21°C and Mounting-head-temperature - 18°C.

I hope you will solve your problem.

Kind regards

Stefan

Thank you everyone, I turned off the whole this and let it thaw, so its all good now.