I'm a structural biologist by using Cryo-EM.

My protein always form a stable complex (GPCR-antibody). But on cryo-em grid, my particles seem to be dissociated.

I use a 200kV microscope comprising of K3 detector. And, I usually collect micrographs at 60 total dose.

I have a question. My total dose usually high or low?

What is the relationship between total dose and particle dissociation?

If reducing the total dose, expect to strongly form the complex ?