Anybody tried this antibody(ab174651) on mouse cells (WB)?
Hi, I am getting elevated results in my starch analysis. It is about 2-3 % higher than usual. We have not changed anything. We are getting the same results over and over. I have checked the...
25 March 2024 1,395 6 View
As an alternative to looking at the conservation of cysteine residues amongst homologs of a protein of interest, is anyone aware of a server that can predict whether disulphide bond formation...
21 March 2024 3,803 1 View
I am trying to purify a his tagged protein using IMAC. The homologs of this protein purify very well. However, this protein precipitates on elution. Buffer composition- 20mM Tris-HCl pH7.4, 500mM...
20 March 2024 9,095 4 View
I have purified several his tagged proteins using IMAC. To clean up the samples further I ran them through a GF column (Superdex 200 increase 10/30Gl). When I ran an SDS sample containing the...
19 March 2024 4,202 3 View
Hi, Has anyone got a method to test glycoalkaloids in potato. I don't have an HPLC so I'm looking for a wet chemistry method. I would like to quantify the results. If possible, I want to look a...
13 November 2023 1,668 1 View
We use an I house sample as a control sample (set up by running a PT sample along side it). However, over a few months it becomes rancid and we have to start the process again. Does anyone know of...
27 July 2023 8,679 0 View
Currently exploring similarities around the Design Thinking, Inquiry Learning, and Mathematical Modelling framework.
17 May 2023 8,746 1 View
as stated
18 February 2023 7,264 1 View
Folks, Does anyone know of a paper showing binding affinity data of DNA oligos for their complement? Ideally it would show data from a 'non-molecular biology method' such as ITC, SPR, or MST. Even...
12 January 2023 2,965 2 View
Can you use the chi-squared test if subjects from the groups in the predictor variable can appear multiple times in the response variable categories? & Can you use chi-square if you do not...
21 July 2022 7,061 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
