How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

CAT assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional catalase enzymatic assay in a 96 well microplate, for cell line and/or C.elegans spectrophotometric measurements. Thanks!

11 February 2021 7,273 3 View

Could someone help me troubleshoot my NOS assay protocol for skeletal muscle extracts, please?

I am trying to measure differences in eNOS activity in skeletal muscle in sedentary and exercised mice, following the conversion of 3H-arg to 3H-cit, and assaying the samples with and with out...

31 January 2021 8,324 1 View

Can anyone recommend me a valid primer sequences of Human Catalase (CAT) for Real Time PCR (SYBR Green)?

Hi all! I'm planning a Real Time PCR (SYBR Green) to to determine the amount of expression of Human Catalase (CAT) in human cardiomyocyte. I compared some primers found in the literature but none...

19 January 2021 2,233 5 View

What is the recommended tool for quality assessments of case reports?

I am performing a systematic review and the majority of published papers are case reports. In the same way we use the Newcastle-Ottawa scale (NOS) to evaluate the methodological design quality of...

13 September 2020 2,373 5 View

What is the recommended tool to assess the quality of case-control studies included in a meta-anlysis?

I am working on a meta-analysis of case-control studies. I used Newcastle-Ottawa scale (NOS) to assess the quality of the studies. I couldn't find a good reference to interpret the results...

08 March 2020 7,961 4 View

In a one thesis:kit ( solar bio human superoxide dismutase assay ) plaese I want steps of procedure in detail , the formula and how draw the curv ?

3.2.7.2.4.3 Serum superoxide dismutase (SOD) The ELx800 absorbance micro plate reader from BioTek (USA) instruments was used to measure the absorbance of SOD in serum. The solar bio human...

10 November 2019 6,510 2 View

I'm currently working on a project that has a lot to do with uniformly convex spaces. Apart from Hilbert, Lp, lp, and CAT(0), is there any other?

I'm currently working on fixed point theorems on uniformly convex spaces and I will love if anyone can point my attention to spaces that are uniformly convex apart from the ones I have listed above.

22 September 2019 1,351 8 View

Can anyone help with the best - Classroom Assessment Technique (CAT)?

Classroom assessment is a way of helping teacher/lecturer obtain useful feedback on what, how much, and how well their students are learning. Can anyone help with best Classroom Assessment Technique?

04 May 2019 4,576 14 View

What should be the Protein concentration for assays?

for CAT activity, is there is any threshold regarding the concentration of protein in the enzyme has to be taken into consideration before doing the experiment.

07 March 2019 3,109 5 View

Does anyone have experience using the Chat-Cre mice lines from Jackson? I am having trouble getting the PCR bands to show up during genotyping?

'GCA AAG AGA CCT CAT CTG TGG A' - WT Forward Primer 'CAG GGT TAG TAG GGG CTG AC' - Common Primer 'CAA AAG CGC TCT GAA GTT CCT' - Mt Forward Primer PCR Protocol is attached. In brief, used as low...

02 March 2019 6,902 6 View