Hello, for my graduation project I have to compare cells in a 3D- Model to one another in regard of their volume, side faces and the corresponding forms and their numbers.
First, I have about 300 Tif-Files of microscopic planes of cells (black and white) that I want to create a 3D-Model out of orientating on the cell membranes.
Second, I need a code or program that can differentiate the cell membrane (white to grey in TIF) from the cytoplasm (black in TIF), kind of trace it and then analyze the volumina of each cell and side faces in regard to their forms (what types of polygons they resemble) and the number of each form in the whole 3D-Stack (not bound to the cells where they occur, just a numeric listing).
Can you recommend a program for these problems? Or for the individual programms?
I am unfortunately not very knowledgeable in this field and would greatly appreciate your advice.
To create a 3D model from a 2D stack of microscopic planes, you will need to use a 3D modeling software such as AutoCAD, Blender, or Solidworks. Once you have imported the 2D stack of images, you can then use the software to create the 3D model. This will involve extruding the 2D planes into 3D shapes, connecting them together, and adjusting the overall geometry. Once the 3D model has been created, you can then analyze it according to volume, side faces, and number. To measure the volume, you can use the software's built-in tools to calculate the size of the model. To measure the number of side faces, you can simply count the number of faces that make up the model. To measure the number of faces, you can use the software's built-in tools to calculate the surface area of the model.
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