Covid-19 survey - FAQS.TIPS

More Milon Selvam Dennison's questions See All

How to avoid background signal in screen printed electrode?

Hi, I am doing aptamer based electrochemical biosensor. First, I cleaned screen printed gold electrode using 0.5M H2SO4. Then, immobilized aptamer followed by blocking using 2mM MCH. I use...

06 July 2024 9,852 1 View

How to filter/get the silver nanoparticles &nanoplates ? We synthesis this silver nanoparticles materials from the NaBh4, trisodium citrate,H2O2?

After finish the synthesis of nanoparticles.and nanoplates of silver how to take out those as solid/powder form from the solution. Please give a solution of u already done that and succeeded by that

27 June 2024 692 1 View

What is the most convenient way to pollinate tomatoes?

In terms of labor, cost, and pollination efficiency, what is the most convenient way to pollinate tomatoes grown under controlled environment agriculture facilities, especially in greenhouses?...

18 June 2024 3,825 2 View

Autodock Vina Grid Spacing is 0.375 A or 1.0 A?

Hello everyone, I am conducting molecular docking using Autodock Vina. The receptor (protein) is huge therefore the grid spacing 0.375 A is not enough (Autodock Tools). So, I also increased the...

22 March 2024 8,368 1 View

Mental Health Professionals, can you fill out this form for my research purpose?

I, Prasanna P, am currently pursuing my final year of B.Sc.Psychology at Thiagarajar College of Arts and Science, Madurai. I am doing research on "The Role Of Inner Speech In Fostering Resilience...

24 December 2023 1,675 2 View

What makes high 260/280 ratio for purified protein?

Hi everyone, I have expressed 6x histidine tagged protein(35kDa) and purified using Ni-NTA agarose (Qiagen). The elution buffer contain 20mM of Sodium phosphate, 500mM NaCl and 250mM Imidazole....

20 December 2023 7,081 1 View

Why Lemo21 E.coli did not grow on LB broth after subculture from overnight culture?

Hi everyone, I am currently working on protein expression and purification. Transformation was successful using Lemo21 E.coli and plasmid pRSETA. Single colony was inoculated into 100mL LB broth...

18 November 2023 137 6 View

Can we perform docking the structure of two ligands?

Can we perform docking the structure of two ligands? For example can we dock the structure of ZnO with the structure of any compounds of plant using Gaussian software? If we can do how to perform...

20 July 2023 2,889 3 View

How to fix the error "The processing of the last link ended abnormally. All processing has been aborted" in Gaussian software?

I am working on optimizing zinc oxide using gaussian, but am getting the error "The processing of the last link ended abnormally. All processing has been aborted". How to fix this error? I used...

02 July 2023 5,873 5 View

Which structure of ZnO can we use to compare Theoretical work with experimental work?

Can we do the computational studies of ZnO Nanoparticles using Gaussian Software? If yes, Which structure of ZnO can we use to compare Theoretical work with...

29 June 2023 802 3 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

A paper on a fossil lycopod?

Dear all, Does anybody have a pdf of this paper? Wood, G.R. and Beeston, J.W., 1986. A late Permian lycopod cone Skilliostrobus sp. cf. S. australis Ash 1979 from Queensland. Geological Survey of...

07 August 2024 4,857 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

E-commerce businesses, COVID-19 and Entrepreneurial Strategies (innovation, proactiveness and risk-taking)?

Hello: I am looking for articles that address e-commerce businesses and COVID-19 survivors, as well as Entrepreneurial strategies that reflect e-commerce businesses. Blessings Evelyn Dempsey

05 August 2024 5,332 3 View

Why is the molecule's orientation with an electric field affect polarizability?

Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...

03 August 2024 7,843 1 View

Why do paired electrons atoms produce no magnetic field lines?

If you think of electrons with spin as bar magnets, you know bar magnets of opposite polarity as long as they're not occupying the same spatial location don't cancel out each other's magnetic...

03 August 2024 3,927 2 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

I am looking for the permission to use the OLBI survey. Does anyone know where I can obtain that information?

I have reached out to the original creator Dr. Demerouti but have had no luck with a response. I am completing my IRB form and need the information or permission by tomorrow. I know short notice,...

29 July 2024 3,684 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View