I want to observe chemotactic movement of e coli using fluorescence microscope. DAPI staining protocol didn't work for me and syto 9 is too expensive.
Thank you so much. I tried using DAPI twice, and both times it didn't stain properly, even with concentrated DAPI solution. Thanks 😁
For my future discharge forecast in the calibrated HEC HMS model, i need the precipitation data of the future.How can i get this data in the daily timescale?
21 December 2020 8,928 5 View
My study area of rainfall-runoff modelling is in mountainous region and has snow cover. There is about 10 percent snowmelt contribution to the discharge of the river i am modelling. How can I...
23 November 2020 7,873 4 View
I dont have any other soil maps for my study area.. How can i use this data to convert it into Hydrological soil groups A,B,C or D ?
04 August 2020 7,848 6 View
I need numbered APA style using square brackets for in-text citation. And, also slight modifications on the standard APA Bibliography format. How do you modify in-text citations as well as...
23 December 2019 5,692 5 View
Phosphorus release higher when pH adjusted with HCl acid at pH5 but Phosphorus release less when pH adjusted with bubbling air though the pH5.
19 September 2019 1,251 5 View
I plan to work in greenhouse to see the effects on soil microorganism.
23 August 2019 3,361 1 View
Hi, I have worked with onion epidermal cell transformation for a long time now. Did some successful transformations with high efficiency towards the end of last year and beginning of this year...
19 October 2016 9,411 7 View
I want to remove lipids from the DCM extract of soil and plant samples for the analysis of PAHs. For this I can not use Conc. sulfuric acid as these compounds are sensitive to possible sulfonation.
25 July 2016 4,992 3 View
as far as i know arrhenius equation is derived on the dependency of rate on temperature but the question above is valid and has been asked many times?
18 November 2015 2,324 2 View
I have been working on extraction of mRNA of a gene of my interest in Arabidopsis. However, I am confused with what to expect in the gel after i run Total RNA (meaning mRNA, tRNA, rRNA) in a gel....
26 October 2015 7,581 3 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View