I want to observe chemotactic movement of e coli using fluorescence microscope. DAPI staining protocol didn't work for me and syto 9 is too expensive.
Thank you so much. I tried using DAPI twice, and both times it didn't stain properly, even with concentrated DAPI solution. Thanks 😁
Hello connection !!!!! I am currently studying MSc. Statistics in Tribhuwan University. I want to do thesis in the topic of Bayesian Inference . Can you share me the probable topic for my thesis.
17 July 2024 2,590 1 View
Forecasting using ANN for a single variable. Say, inflation.
03 April 2024 6,529 4 View
I am checking if there any systematic differences in physical activity among different income group and education level for my masters’ thesis. The physical activity has been assessed through...
27 February 2024 5,859 2 View
If Census was done, will the sample size below 40 acceptable?
15 August 2023 5,048 6 View
I want to know the fee required for publishing a original research paper. Thank you.
03 June 2023 4,161 1 View
I need some of the research paper related on this.
16 April 2023 6,619 1 View
I am unable to calculate the power for the circuit design in the Sentaurus TCAT. Suppose I have designed the NAND gate by using the FinFET and GAAFET. in Sentaurus TCAD. Now I want to calculate...
16 December 2021 2,216 1 View
I treated WT mice with oxonic acid/Vehicle for four weeks and measured uric acid from serum using uric acid assay kit from bioassay system (DIUR-250). To my surprise, the uric acid value was...
01 June 2021 9,719 0 View
I contacted Sigma Aldrich technical support for oxonic acid potassium salt solubility, and I was informed that it dissolves in water (60mg/ml). I tried in water with constant heating and stirring...
07 April 2021 2,932 2 View
For my future discharge forecast in the calibrated HEC HMS model, i need the precipitation data of the future.How can i get this data in the daily timescale?
21 December 2020 9,259 5 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,337 2 View
How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?
07 August 2024 2,132 3 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,727 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,706 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,973 3 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,733 1 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View