I am going to carry out an experiment to separate and extract the ssDNA obtained from nicking endonuclease-treated plasmids. However, I had no idea how to prepare denaturing agorose gel containing urea. I wonder if you could share some recipes. Additionally, I've read one article using urea containing TAE buffer as well but I am concerned how urea could influence electrophoresis rate and how to control electrophresis temperature. I'd be most grateful if you could help me!