I am looking for an in vitro herbicidal activity test for prospective bioactivity purposes. I would like to use an in vitro test for a first step screening, but I do not have experience in this field. Any suggestion is welcome. Thanks you very much
In STATISTICA 13.3, I used the Experimental Design Builder under the Design of Experiments tab to generate a design matrix for an asymmetrical factorial design (1443) with 12 experiments. However,...
29 July 2024 4,155 2 View
Much is said about AI and the Communication field. Some say that Communications would very much benefit from that intersection. But specifically which areas would benefit from AI? Could it be a...
11 July 2024 5,335 4 View
Hi everyone! So I am trying to concentrate my recombinant proteins in order to make some assays with them, but I need a "considerable" concentration of each. One of my proteins is around 9kDa and...
08 May 2024 5,418 2 View
Dear all, My question is addresses to reliability regards how reviewers or journal editors would take it when they realize that the methodology of a paper was mainly done through this software. I...
15 April 2024 9,305 0 View
I'm encountering difficulty in achieving a purified extract of erythrocyte membrane proteins using a lysis procedure with 0.1% Triton, followed by multiple centrifugation steps at 12000 g and...
03 April 2024 7,725 2 View
I would like to measure the oxygen consumption rate directly from tissue in the seahorse XFe96 analyzer with the Seahorse XFe96 Spheroid FluxPak (Part Number:102905-100) but i need a specific glue...
02 April 2024 8,718 1 View
Does anyone know what is the refractive index of MoSi2 and in what medium it can be performed (distilled water or ethyl alcohol)? I pretend to perform a particle-size distribution analysis by...
23 March 2024 6,736 1 View
Does anyone know where to purchase research-grade Galleria mellonella in Europe? I only find the TruLarv from BioSystems Technology, but it seems to be down... Thank you!
11 March 2024 4,800 2 View
Dear Forum Members, I hope this message finds you well. I am currently considering the purchase of a Q-TOF LCMS-9030 Mass Spectrometer for my laboratory, and I would greatly appreciate hearing...
10 March 2024 9,081 0 View
I am conducting research on the performance of primary school teachers who use AI, and I have not been able to find a validated instrument that fits this situation. I would like to hear your...
22 February 2024 6,052 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...
26 July 2024 6,606 0 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
What is the heating temperature that I can use to deactivate enzymes in bacterial extract exopolysaccharide without loss of the bioactivity of EPS? Hi I am trying to make the purification (by TCA...
22 July 2024 5,334 6 View