Could you please suggest a technique of a plant root preparation for XRF confocal imaging?

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Olena Ponomarenko

A specifics of the beamline used plays a big role. What is perfectly fine for a partciluar beamline/source may not work for another. The fine details are not always found in the published literature.

Joseph R. Chidiac

It seems embedding your sample in paraffin wax and taking longitudinal sections might be the best way to view the root internal tissue by confocal microscopy.

I hope this helps, and I wish you success in research.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4698609/