I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed 96 wells microplates while others suggest using flat-bottomed plates. I would like to have your thoughts on whether this could impact the MIC results as well as the process of MIC reading using either naked eyes or microplate readers. As for the treatment, I have read one particular article ( Article Effects of Microplate Type and Broth Additives on Microdilut...
) comparing treated and untreated plates, recommending to use nonspecific binding(NSB) plates as opposed to adding surfactants when testing lipophilic agents. That said, I would like to have your practical experience on whether the use of BSB plates, as well as the addition of surfactants, would apply to all lipophilic agents.Thanks in advance
Solomon Abrha
Thank you so much Subas Chandra Dinda and Shaista Bano for your responses.
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