Could you please share protocol to culture primary macrophages from synovial fluid/tissue of Rheumatoid Arthritis patients.
And it will extend up to how many passages.
thanks
Macrophages do not divide in culture so there is no point in trying to passage them. Synovial fluid and synovial tissue contain various macrophage populations in different states of differentitation. The proportions will vary enormously from sample to sample. I doubt that anything very useful would come from trying to study their properties in tissue culture to be honest. Is there some particular reason for studying macrophages from rheumatoid joints (which are hard to get samples from anyway) rather than studying macrophages derived from normal monocytes? It is unlikely that there is anything inherently different about the macrophages of RA patients and many of those in synovial fluid are probably moribund.
I agree with Jonathan. Further, upon culturing, oxidative stress is likely to activate p53 pathway and other stress pathways. Therefore, any information you may get will likely be an artifact of cell culture.
thanks for your suggestions sir. Actually our work is on RA patients and we use to get the samples from AIIMS. We already cultured synovial fibroblast from synovial fluid of RA patients. Now we want to extend our study to macrophages also. Thats why i need the macrophage culture protocol.
thanks
Dear Jyotsna,
As I indicated, you cannot culture macrophages like fibroblasts. They do not grow. It would still be useful to know why you might want to study them in culture at all. I am very doubtful that you can answer any useful questions by doing so.
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