I want to measure soil microbial respiration in a laboratory, and I' will appreciate it if you can provide me with simple experimental procedures that aren't kits.
Soil MBC of air-dried soil and moist soil are highly correlated and are similar in values (Franzluebbers et al. 1996, Mondini et al. 2002) if measured after about 2 days pre-incubation. Therefore, the sieved (
First, averag basal respiration can be measured for 48 hours. Then microbial biomass would be determined by the Substrate-Induced Respiration method (Anderson and Domsch 1978)whereby the increase in respiration rate over 24 hours after addition of substrate (0.22 g glucose /pot) would be used to calculate the amount of microbial biomass C in soil (mg C/100 g DM soil). Best regards