Can we use two antibodies generated in same species for indirect Sandwich ELISA?

I have generated two antibodies against two peptide fractions of a protein in the rabbit. I want to use these two antibodies (Ab-1 and Ab-2) in indirect sandwich ELISA for the detection of...

11 January 2021 1,754 4 View

How to coat plasma sample (Antigen) in ELISA plate for a direct ELISA?

I have generated an antibody against an antigen. Now, I want to use the developed antibody in the detection of the unknown sample. So, I want to coat the plasma sample as antigen. How that can be...

26 October 2020 2,259 7 View

Which path loss model will be suitable considering the scenario of factory automation with 5G URLLC?

In the performance analysis of modern wireless communication systems like the scenario of factory automation with 5G URLLC, which path loss model will be suitable and appropriate to assume? Any...

03 July 2020 918 2 View

How can i ensure the complete oxidation of graphite before quenching the oxidation process by H2O2?

I am following KMnO4 -H2SO4 reaction media for the synthesis of graphene oxide(GO) by an improved process.

04 June 2020 508 3 View

Does the SIMPLE algorithm solve the VOF single momentum equation as the descritized momentum equation, in free surface flow?

What is the link between VOF model and SIMPLE algorithm in CFD modeling ? How is the computing cycle works ?

10 February 2019 1,445 3 View

ALT and AST during hepatic damage - ELISA or Kinetic method ?

ALT and AST is released into the circulation during the hepatic damage. And hence, ALT and AST levels are reliable method to determine the hepatic damage. What is the drawback in using ELISA...

12 November 2018 5,636 1 View

How 5G Wireless Network can be Deployed with Existing 4G Infrastrucure?

As Time moves us towards the 5G Wireless Network...Needs Solution for Low Cost Technology for its Implementation.

02 May 2018 5,188 6 View

Hai ..any body please suggest OA journal with less fee and fast acceptance. I am totally confused with a great big list of open access.?

I need a good very less fee open acces (SCI or scopus) with good IF with less first review decision and too less rejection rate (sorry for giving more requisite) .I am from very poor back ground...

09 March 2018 9,141 5 View

DNA Ladders - Cheapest Option?

Dear Fellow Scientists, My lab just ran out of DNA ladders (and my student only told me today). The ordering process takes a long time in Sri Lanka and I'm wondering what would be the best bet for...

16 March 2017 3,543 3 View

How to pack fruit plants (EX .Avacado,Mango)to transport a long distance without having soil media.?What is the procedure..?

Here I am expecting the packing procedure to send to another country.

01 February 2017 1,100 3 View

Did any urologists have experienced with retroperitonel leiomyosarcoma from renal vein with IVC enxtension?

Recently, we got one lady with right side retroperitoneal mass involving renal vein and extends into IVC upto diaphragm. CT suggested leiomyosarcoma. Right kidney separately seen from mass and it...

19 February 2021 6,873 1 View

Wave velocity and rock density paradox?

Practically it is known that seismic velocity increases as rock density increases, why then does the wave velocity equation involving elastic moduli and density indicate that velocity is inversely...

09 February 2021 1,843 3 View

[Covid-19] GGO Evaluation - CT imaging of the lungs ?

13 January 2021 3,115 4 View

Where can I find a dataset for kidney stones images (CT or ultrasound)?

I want to apply neural network on kidney stones images (whether its CT images or ultrasound) to determine whether the kidney has stones or not.

02 January 2021 9,938 3 View

How can the moment tensor inversion results can present good waveform fit (synthetic and observed waveform) from generalized Cut and Paste scheme?

Depending upon the distance of the seismic station from the epicentre, the quality of waveform data and the improvisation of the synthetic waveform on it is often a question. If the Pnl waveform...

31 December 2020 6,602 2 View

What is New Next Generation Attenuation Relationships (NNGAR) and how are they different from other attenuation relationships?

Dear researchers The provisions of ASCE 7-10 states that New Next Generation Attenuation Relationships (NNGAR) has been used in the process of Probabilistic Seismic Hazard Analysis (PSHA) to...

15 November 2020 1,132 4 View

Selection of Steel Frame Section in SAP2000?

Hello, I have a technical question about the selection of steel frame sections in the designing of steel frame structure i.e W16x48 etc. The common practice I have seen is the trial and error...

11 November 2020 5,762 4 View

Can anyone please tell how to define link for Tuned mass Dampers using Sap2000/Etabs?

Dear Research scholars, I was doing my MTech thesis on pendulum based TMD and I was having trouble defining the link properties. I even followed the tutorials put on CSI website. still, I wasn't...

11 November 2020 8,309 11 View

Sir can you send me your research paper " Earthquake risk management assessment and evaluation for turkey" in short time?

05 November 2020 10,019 3 View

How i can extract the dose delivered to each pixel of CT image of TPS?

I generated a treatment plane for a patient using intensity modulated radiation therapy (IMRT). I intend to calculate cell survival in each voxel of target volume using LQ model. So, i need to...

03 November 2020 6,132 3 View