Design a taqman primers and probes for gene expression assay?

dear all as it is my first time to design a taqman assay , could you recommend for me a good reference to know the good designing for taqman gene expression assay, what is the accepted range for...

15 May 2019 8,287 2 View

Can Chloroform be used as a solvent in cytotoxicity test in cell culture instead of DMSO ?

my drugs can not be dissolved in any other solvent except the Chloroform, so can I use it on cells in MTT cytotoxicity test

09 December 2018 4,922 4 View

How can I estimate the shape and scale parameter of the Generalized Pareto Distribution (GPD) given a specific data-set?

I have already estimated the threshold that I will consider, created the pdf of the extreme values that I will take into account and currently trying to fit to this a Generalized Pareto...

18 May 2017 5,083 3 View

Best way to extract RNA from FFPE human brain tissue?

I am trying to compare yield of fresh vs FFPE and the measure relative quantity by RT-qPCR of housekeeping gene. I can upload the protocol I am using right now if anyone is interested in sharing...

11 December 2016 852 2 View

Should I use isolated RNA or purified mRNA for RT-PCR?

Will isolate RNA from tissue. I will not be running RT-qPCR, but Reverse Transcription PCR. For qPCR typically RNA is used instead of mRNA, I wonder if the same applies to RT-PCR. I only want to...

15 May 2016 3,985 6 View

Any suggestions for Online Learning Libraries in C++?

To be more accurate, I need Online Regression Libraries implemented in C++ (as I want to integrate them into a C++ project). So far I have found sofia-ml and OnlineSVR. Any other suggestions?

08 July 2013 9,397 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View