We were using oil red stain to stain fat droplets in adipogenic differentiated MG63 and now we are trying new nile red stain.
Thank you
Nile red staining procedure is very much straight forward. We use it to stain Lipid Droplets in zebrafish embryos. It works for both live/fixed cells. Prior to staining the cells are washed with PBS 2-3 times. For cells, 300nM of Nile red is sufficient. The cells are incubated with nile red solution for 10-15 min. The cells are then washed with PBS to remove excess dye. This is followed by imaging under green excitation light.
you can also go through the following paper. It was the first one to report the use of Nile red for staining Lipid drplets
https://www.ncbi.nlm.nih.gov/pubmed/3972906
Nile red staining procedure is very much straight forward. We use it to stain Lipid Droplets in zebrafish embryos. It works for both live/fixed cells. Prior to staining the cells are washed with PBS 2-3 times. For cells, 300nM of Nile red is sufficient. The cells are incubated with nile red solution for 10-15 min. The cells are then washed with PBS to remove excess dye. This is followed by imaging under green excitation light.
you can also go through the following paper. It was the first one to report the use of Nile red for staining Lipid drplets
https://www.ncbi.nlm.nih.gov/pubmed/3972906
please look at technical notices or previous publication(s), such as the one noted above
Googling (or any other database ) for:
| "Nile red stain*" AND lipid* AND recipe | [ca. 680 results] or expanding to:
| Nile red stain* AND lipid* AND recipe | (over 1 Mio results - the first 30-40 results are sufficient and will help you extremely!.....(knowing that you might not be able to use Google in your country....I apologize)....
My best wishes and good luck.....
- Badges
- Science method
- Similar topics
- Biological Science
- Histology
- Staining