Hello everyone

Here, I mean there is a high level of familiarity between VIM-1 and VIM-2 and I'm not sure if I can detect these two genes and distinguish them in Pseudomonas aeruginosa isolates by PCR.

I have blasted some primers—from articles—for these genes in NCBI and for example, I saw a primer pair in 80% of cases bonded to VIM-1, but unfortunately, the rest of the targets were sequences other than VIM-1, like VIM-2 and...

Do I need some other test like sequencing?