I want to make a endothelial barrier on a transwell with high TEER, and MDCK type I's are known to have very high values, so I was looking for suppliers. However, ATCC doesn't carry them, and ECACC directs me to Sigma. Would anyone know other suppliers of these cells?
In the Book: Ion Channel Localization: Methods and Protocols
edited by Anatoli N. Lopatin, Colin G. Nichols, the authors are mention MDCK type I cells. Maybe you can see where the authors they are citing got the cells from. Link: https://books.google.es/books?id=PCvZEZ5fYu8C&pg=PA190&lpg=PA190&dq=MDCK+type+I&source=bl&ots=2BdLnO3ioJ&sig=zUrwi_D_5oTx3XHYF0Na_MOh5ww&hl=en&sa=X&ved=0ahUKEwii1-W5tqTTAhVG1RoKHZ8_BlgQ6AEIVjAG#v=onepage&q=MDCK%20type%20I&f=false
Dear Xiaoling,
You can check following publications of MDCK cell lines for further help:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3209442/
https://www.ncbi.nlm.nih.gov/pubmed/9874698
Regards!
Thank you all for the suggestions!
I have collaborated before but I was wondering if there may be other suppliers I'm unaware of as well. I'll look at other literature that use MDCK type I cells and try to find out where they obtained theirs...
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