Hi all,
I am trying to stablish a phylogeny of 24 species of enterobacteriaceae I have (only for presentation purposes in my figure, and NOT a serious phylogenetic anlaysis), but my 16s rRNA sequences seem to give rise to a tree that does not agree with the order of genera and I cannot find a suitable paper on this topic.
A multi-gene/protein phylogeny would be great for me to compare my tree and configure my tree accordingly.
Thank you.
Did you construct the trees by different methods, such as NJ, ML and Bayes?
I did, but even the ones by the same methods gave different results. The problem is that 16s does not evolve fast enough to provide any reasonable comparative differences and hence is useless for constructing a phylogeny for these organisms.
I hope this help.
http://www.biomedcentral.com/1752-0509/7/46/figure/F1
Article Inferring ancient metabolism using ancestral core metabolic ...
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