We have been trying to isolate essential oil from a tree bark. However, the yield is very less which is making it difficult to quantify the yield and for further analysis.
Did you powder the bark before distillation? Soak the powder in distillation water overnight and check if it improves recovery. In other threads, suggestions were given to add glycerol or n-pentanol to increase recovery. As far as recovering the oil from hydrosol is concerned, ethyl acetate, n-hexane, n-butyl acetate, n-pentane, diethyl ether, ethanol, cyclohexane, methylene chloride (dichloroethane) etc. were used by different researchers to recover the essential oil. Check the following threads for methods of essential oil isolation as well as isolation of essential oil from hydrosol.
Similar problem occurs with vetiver and some Ocimum species.
https://www.researchgate.net/post/What_extraction_procedure_is_best_for_the_analysis_of_the_essential_oils_from_plants
https://www.researchgate.net/post/How_can_we_prevent_froathing_during_extraction_of_essential_oils_by_hydrodistillation_using_clevenger_type_apparatus
https://www.researchgate.net/post/How_do_I_extract_essential_oil_from_fresh_leaves_in_a_short_time
https://www.researchgate.net/post/how_to_estimate_the_volatile_oil_content_in_oleoresins
https://www.researchgate.net/post/How_do_I_treat_the_residual_distillation_waste_water_of_essential_oil
https://www.researchgate.net/post/What_solvent_and_concentration_is_suitable_to_solve_volatile_oil_in_water_without_affecting_germination_and_plant_growth
https://www.researchgate.net/post/What_is_a_suitable_method_for_extraction_of_highly_volatile_compounds
The first thing I know is that method are used for obtaining essential oil. I imagine you're doing by hydrodistillation. The second point wanted to know what kind of isolating desired compounds, if volatile, semivolatile or vapor pressure compounds reduced. When you suministres me this information, I can recommend an appropriate method of extraction.
As for the method of concentration, what I recommend is to use a rotary evaporator under reduced pressure, and less than 40 ° C, to avoid decomposition of thermally labile compounds.
I am attaching some articles that you may find helpful.
Dear Shyam Suwal,
I used a Likens-Nickerson apparatus to extract volatiles from several plant species. As solvent I used distilled n-pentane. You can concentrate it using a rotary evaporator or under a continuous nitrogine flux.
You can see that in this paper
http://www.sciencedirect.com/science/article/pii/S092666901100029X#
Good luck.
n-hexane or petrolium ether, then concentration under reduced pressure less than 40 C
Thank you very much for your help. Actually we are using hydro and steam distillation process. Due to the low yield the isolated essential oils are dispersed in the hydrolsol (water phase). We actually want to recover these dispersed essential oil. Therefore, I wanted to know if there is any possibility to trap them in any solvent and eventually concentrate them. Thank you.
Did you powder the bark before distillation? Soak the powder in distillation water overnight and check if it improves recovery. In other threads, suggestions were given to add glycerol or n-pentanol to increase recovery. As far as recovering the oil from hydrosol is concerned, ethyl acetate, n-hexane, n-butyl acetate, n-pentane, diethyl ether, ethanol, cyclohexane, methylene chloride (dichloroethane) etc. were used by different researchers to recover the essential oil. Check the following threads for methods of essential oil isolation as well as isolation of essential oil from hydrosol.
Similar problem occurs with vetiver and some Ocimum species.
https://www.researchgate.net/post/What_extraction_procedure_is_best_for_the_analysis_of_the_essential_oils_from_plants
https://www.researchgate.net/post/How_can_we_prevent_froathing_during_extraction_of_essential_oils_by_hydrodistillation_using_clevenger_type_apparatus
https://www.researchgate.net/post/How_do_I_extract_essential_oil_from_fresh_leaves_in_a_short_time
https://www.researchgate.net/post/how_to_estimate_the_volatile_oil_content_in_oleoresins
https://www.researchgate.net/post/How_do_I_treat_the_residual_distillation_waste_water_of_essential_oil
https://www.researchgate.net/post/What_solvent_and_concentration_is_suitable_to_solve_volatile_oil_in_water_without_affecting_germination_and_plant_growth
https://www.researchgate.net/post/What_is_a_suitable_method_for_extraction_of_highly_volatile_compounds
Thank you so much B.R. Rajeswara Rao for your suggestions and comments. Yes we powdered the bark materials before the extraction.
Try with non-polar solvent such as Petroleum ether, hexane, diethyl ether etc.
Do you have facilities for super critical extraction, that is the best as the there is no solvent effect when carbon dioxide in used as the super critical fluid.
If not I recommend to use diethyl ether and rotary evaporator can be used to evaporate the solvent.
I think we are all saying the same thing as long as the solvent is very volatile and in-miscible with water.
bonjour
je vous propose d'extraire votre écorce en poudre par sonication et de re-extraire par hydrodistillation
Thank you very much for your kind help. Really appreciated. I have another question. When we fractionate essential oil with solvent, how can we ensure that there is no trace of solvent left after the evaporation? Thank you again.
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