I'm following the protocol suggested by J. Biol. Chem.-2004, Levenson et al (attached), however, they don't say the volume of Tris in the end of the protocol.
I want to know more about Uranium ore deposits in world.
11 August 2024 6,720 0 View
I want to know more about diamond ore deposits in world.
11 August 2024 2,167 1 View
We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...
10 August 2024 6,678 14 View
If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?
10 August 2024 8,198 5 View
I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
10 August 2024 3,229 0 View
What are a “Farmers Producer Organization” (FPO) and its essential features?
10 August 2024 477 5 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...
08 August 2024 4,831 4 View
How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?
08 August 2024 8,162 0 View
If they can be acetylated and methylated what will be the effect?
02 July 2024 4,030 2 View
Cytosine methylation, histone acetylation, etc. represent distinct epigenetic mechanisms of gene regulation. Which example is most likely to account for transgenerational effects, such as...
31 May 2024 4,613 3 View
Hi all! I would like to know if there is a method to polymerize low MW cellulose triacetate (e.g. re-engaging in acetylation). Or this is not possible and the MW of my cellulose source should be...
08 May 2024 5,260 1 View
Can epigenetic modifications affect various aspects of sperm development, function, and fertilization?
27 April 2024 6,902 2 View
Basically, I have deprotected a BOC, TPDMS-Protected sphingolipids using Acetyl Chloride, which gave a salt of deprotected sphingolipids. Can salts run on TLC?!
25 April 2024 2,971 1 View
Dear all, I have performed CUT&TAG and tried to pulldown H3K27me3 containing nucleosomes in the cells treated with EZH2 inhibitor. I spiked my human cells with 20% of Drosophila cells. As...
21 April 2024 3,197 0 View
I'm new to epigenomic analysis and have confirmed the high chromatin accessibility of a gene in tissue cells via ATAC-seq. However, I'm unsure about the specific histone modification or DNA...
13 April 2024 7,086 0 View
Deacetylation of chitin results in different de acetyl chitin (chitosan)with various degrees of de acetylation.Such variation produces a mixture of different( chitosans) which is not studied...
02 April 2024 2,739 0 View
I am performing Cut & Tag experiment. I was wondering if there is any alternative/replacement for TAPS pH 8.5 in single tube Tagmentation and pA-Tn5 release ( DNA purification) step of Cut and...
02 April 2024 4,214 0 View
I am trying to find a correlation between fetal macrosomia and hypertension during pregnancy from CDC data. But there have been a lot of studies on the epigenetics of the underlying relationship...
29 February 2024 7,013 1 View