Could someone help me with a problem occuring in RACE PCR 5'UTR ?

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Gregory Dressler

Your 5' UTR may have significant secondary structure such that the reverse transcriptase is skipping a portion of the sequence. If you need to clone this, why not try amplifying that region from the genomic DNA. Alternatively, you may be able to purchase a complete clone from one of the many DNA repositories.

Hwei Huih Lee

Thanks Gregory for the suggestion.