Do homozygous Cre-expressing mice show any problem?

hi, I am generating a loxp-Cre animal line. However, in order to keep my Cre mice colony going I want to reach the homozygous line so there will no need of PCRs. In Jackson labs they warn you...

17 May 2016 1,338 3 View

Do homozygous Cre-expressing mice show any problem?

hi, I am generating a loxp-Cre animal line. However, in order to keep my Cre mice colony going I want to reach the homozygous line so there will no need of PCRs. In Jackson labs they warn you...

04 May 2016 9,481 4 View

Do you know of grants or similar application to get funding to travel to international conference outside the UK?

Hi all, This is not the typical question but since the budget of my PhD is not that big, I am searching for alternative ways to fund my travel to different conference of interest for my PhD (...

03 April 2016 8,523 0 View

Does anyone know an implementation of OnPLS under R environment?

I find that STATegRa implements O2PLS and that plspm implements PLS (path modeling). I am interested in the attached application of OnPLS.

06 March 2016 2,322 2 View

Would you recomend EXOCET assay to quantify my isolated exosomes from endothelial cells?

Hi, I am looking for a way to quantify my exosomes after I have isolated them. I do not have access to a Nanosight (NTA) and although I can do EM this wont let me to quantify them. Would you...

02 March 2016 4,965 3 View

How can I calculate the iodixanol density when I am running an Optiprep gradient density for exosome isolation?

Hello, I am running an Optiprep iodixanol gradient density to isolate my exosomes. With each run I also have a control which I exact 12 fractions and from the protocol it said I need to calculate...

03 February 2016 4,172 2 View

Which housekeeping small RNA is the best for microRNA qPCR of exosomal RNA?

I think that U6 might be able to be used but I am not sure if it is secreted uniformally in exosomes. Would you recommend another housekeeping? Thanks

11 December 2015 9,133 8 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View