I have tried Trizol and Quiagen RNEasy kits to isolate RNA from waterfowl feces (I added influenza virus in serial dilutions to some feces samples as a positive control). However none of the samples was positive on RT-PCR (controls with RNA isolation from same amount of pure virus culture without feces were clearly positive). Does someone have an experience of detection and isolation of influenza virus from bird feces?

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