Thanks for the replies. Dear Yoram Gerchman , the link is not working.
Actually I don't have any experience of working with microbes before. I am trying to make a research proposal regarding bringing chemical changes in some fruit wastes or any other bio-waste without any chemical reaction.I want to use baker's yeast which is commonly available (and economical) and we use for baking. I want baker's yeast to use fruit or any other bio-waste as nutrient and modify it. But i don't know protocol or conditions to grow them like temperature, pH etc. Second part of the question is after growing and interacting with my sample (bio waste) i want to get rid of yeast from the sample so that i can analyze the changes or modification occurred in it.
You will need to define a research question. What do you want to convert to what? If you want to convert bio-waste to ethanol there are many papers and data on the subject, including many open access. Try to define your question more...
As for measuring ethanol and other alcohols, assuming this is your product, one classical method is using gas-chromatogrph. Google this paper for a simple method:
Gerchman et al. 2012, A simple rapid gas-chromatography flame-ionization-detector (GC-FID) method for the determination of ethanol from fermentation processes, Afr. J. Biotechnol. 11:3612-6 (Attached). The same method should work for other alcohols
Article A simple rapid gas-chromatography flame-ionization- detector...
For the time being, i am not looking for any specific conversion. I just want to introduce my sample in the flask containing baker's yeast for a day or two. and then I want to isolate the sample again and check for any changes if occurs.I want to keep my sample in actual form and just want to observe changes only. I don't want to convert them in something new. I couldn't find any paper or procedure for the baker's yeast. and i don't know what are the conditions and how to grow.
The scientific name is Saccharomyces cerevisiae and there is a wast amount of papers and other resources on this organisms. You can also look at the paper I attached and find some media recipe. Also google YPD medium, a common one.