Could somebody advise on the PCR band in non transfected cell while synthesizing cDNA using plasmid specific primer?

08 August 2013 1 7K Report

I co-transfected cell with reporter and effector plasmids and then sythesis cDNA using reporter plasmid specific primer to see the exogenous expression. In PCR reaction I used frward and rverse primer for specific exons to see the patterns of alternative splicing. The problem is that I also got PCR band in nontransfacted sample though the band is not so strong compared to the transfected cell. Could any one suggest me how to overcome this problem?

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Science method

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