Could somebody advise on the PCR band in non transfected cell while synthesizing cDNA using plasmid specific primer?

06 August 2013 1 8K Report

I co-transfected cell with reporter and effector plasmids and then sythesis cDNA using reporter plasmid specific primer to see the exogenous expression. In PCR reaction I used frward and rverse primer for specific exons to see the patterns of alternative splicing. The problem is that I also got PCR band in nontransfacted sample though the band is not so strong compared to the transfected cell. Could any one suggest me how to overcome this problem?

Stéphane Roche

Your cell have an endogenous and homologous gene.

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Science method

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