Could possibly use inactive vaccine for positive control in PCR?

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What is the most common number of epitopes in an multi-epitope Vaccine?

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Jennifer Lynne Bath

Possibly, what is your objective? You could you please provide more details and then i will try to help.

Mohammed Omar Baba sheikh

Thank you Jennifer for your answer, I have inactivated vaccine of foot and mouth diseae, I was test it by PCR. But did not get the band of genome FMD, my question is why inactivated could not use as positive control like live vaccine?

Jason Gummow

Mohammed, as a generalised answer i would say no. This is because depending on how the inactivation was done the genome is either destroyed or removed and hence no template for PCR to occur. Also are you performing normal PCR or RT-PCR? as FMDV is a ssRNA virus.

Thank you so much Janson for clarifying. yes i did perform normal RT-PCR for FMDV.

Pierre Lebon

Yes you can with the flu inactivated vaccine