Could octopus ink make any interference with fluorescent dyes used in qPCR analysis?

05 May 2016 1 8K Report

I am running qPCR analysis of octopus hatchlings, it is difficult to remove the ink sac of all the samples, I would like to know if I can proceed the analysis, without removing the ink sac. Does the ink make any interference with fluorescent dyes?

Romina Henriques

Hi Oscar,

I don't know about the dyes used in qPCR, but I know that squid ink (and chromatophores) can inhibit PCR reactions. You need to make sure the sample is thoroughly clean before extracting DNA, or dilute the DNA (1/100) before using if you can't get rid of all the ink. Hope this helps!

Cheers,

Romina

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