Could anyone suggest a loading control for activated and resting microglía?

09 September 2017 1 9K Report

Hi everybody! We are carrying out an experiment with cultures of microglia. The treatment we apply, changes their conformation, activating them. I was wondering if someone knows a loading control for western blot that we could use, as b-actin concentration of control samples is different from the ones with treatment. We have also tried HSP-70, annexine V, HDAC-1 and LAMIN B1

Marta González

Thank you a lot Christian for your answer. I really appreciate this information and I will take it into consideration

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