Extraction of necessary amount for a qPCR.
Hi to everyone! Does any of you have experience in transfecting siRNA in Jurkat cells and can provide a reliable method? I have both Neon transfection system and RNAiMAX Lipo, but to date none of...
30 June 2024 6,901 4 View
I'm doing my first research and I can't find a way to see the shoreline evolution.
09 May 2024 1,817 4 View
I run an experiment to test the dispersing property of oxalic acid on TiO2 (anatase) colloids, only by mixting the carboxylic aqueous solution with the titania colloid. My results showed that a...
08 May 2024 4,667 0 View
Hi, I would like to set up an experiment with some plants sprayed with an aqueous solution of 200 μM Methyl Jasmonate with 0.025% Zipper surfactant. From the Sigma/Merch website I see I can buy...
05 February 2024 8,534 1 View
For my research, I need visual and/or auditory event related potential, EEG data from normal patients and patients with Alzheimer's disease. Can anyone suggest where I can find them?
29 September 2023 3,831 3 View
In the liquid phase sintering of Tungsten-Iron-Nickel powder compacts, could the presence of carbon (between 0.6% and 1.5%) significantly influence the final microstructure by altering the...
30 July 2023 4,368 1 View
Good morning, everyone! We are doing a spatial analysis to identify the best sites to install centralised upgrading plants. These plants should collect the biogas produced in several anaerobic...
28 June 2023 1,811 2 View
Hello everybody, I'm a master degree student. I'm working with 16S data on some environmental samples. After all the cleaning, denoising ecc... now I have an object that stores my sequences,...
25 May 2023 6,380 2 View
I measured by DLS the size of a TiO2 nanoparticles suspension sample to compare the results against a TEM measurement (5-6nm). As I expected I got different results from the two experiments due to...
24 May 2023 7,592 8 View
I want to use IEEE 14 bus system to integrate two microgrids in the generator buses. My research would be based on Model predictive control to schedule charge and discharge on on BESS of the...
26 March 2023 1,574 3 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
Hello, colleagues. Recently, I have been working on a metal processing simulation with my UMAT in Abaqus. I have outlined the corresponding simulation, but I keep encountering issues that cause...
30 July 2024 7,062 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...
23 July 2024 6,783 1 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...
21 July 2024 4,322 1 View
Is it reasonable to exclude all trials with a blink or saccade in the 150 ms before stimulus onset? As an alternative, would it be better to exclude blinks (after extending them by about 100 ms...
19 July 2024 3,838 0 View
What happens in snapTotal- seq? (Single-cell Total-RNA Profiling Unveils Regulatory Hubs of Transcription Factors https://www.nature.com/articles/s41467-024-50291-3) In this protocol, several RT...
18 July 2024 10,010 0 View
Image Processing Algorithms, Quantum Computing.
17 July 2024 7,958 2 View
