21 May 2019 2 8K Report

For my PhD project I'm struggling to visualise a protein of interest. I tend to use formaldehyde solution for fixing the tissues.

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Can the Pt film that sticks a FIB-prepared TEM foil to its holder be heated up to 180°C in a TEM in-situ experiment without softening?

Hello, we are studying temperature-dependent transformations of a sample containing a hard chrome film. The TEM foil was prepared by FIB because it is the only way to thin the hard chrome. The...

06 January 2021 3,963 5 View

How to estimate the sample size of a hypothetical clinical trial based on principal component scores?

I am wondering whether there is any relevant literature on how to best estimate the sample size of a hypothetical clinical trial with various treatment effects (10%, 20%, 30%) based on the scores...

12 March 2020 9,282 3 View

Quenching PCL autofluorescence at far-red wavelengths?

I am working with polycaprolactone scaffolds and I would like to use in-cell western to determine the protein levels of bone markers. The issue is that even at these far red wavelengths used in...

13 February 2019 3,622 0 View

How to analyze delayed sooner reward options in an inter temporal choice task?

Hello, I conduct an inter temporal choice experiment where each trial consists of two different reward options with different time points. Subjects need to decide for one of the two options per...

08 March 2016 4,348 8 View

What is the best way to calculate delay discounting in an inter temporal choice task?

I conducted a study where the participant had to decide between a sooner smaller and a delayed larger reward option. Across the experiment the delayed larger reward option stayed the same while...

16 February 2016 2,220 4 View

Is there a way to sequentially randomize python files?

I created several psychopy coding files consisting of python language each forming one condition of an experiment. I want that the python files are randomly chosen and each of them is used once. I...

03 September 2015 1,356 6 View

What is a good measure (questionnaire, scale) for hedonic goals /emotional goal focus at the workplace?

I would like to assess to what extent employees are motivated by the emotional satisfaction they gain from their work. Therefore, I thought, a good way to assess it would be by measuring...

24 June 2015 1,657 3 View

Does anyone have any data regarding SOX2, OCT4 and NANOG gene expression of bone marrow mesenchymal stem cells?

I was not able to find any data regarding SOX2, OCT4 and NANOG gene expression of rat bone marrow mesenchymal stem cell. If you can, please share with me :)

20 June 2015 2,293 5 View

How to measure behaviors of aggression vs. behaviors of helping?

I would like to do it in a laboratory setting

25 September 2013 8,338 3 View

Does anyone know research into the impact of psychosocial stress on Graves disease not published in English or German?

There are some preliminary results indicating that there could be an pathogenetic influence of ongoing distress as a trigger for Graves disease. That could create an additional approach to the...

15 December 2012 7,952 4 View

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ANY free software for reconstructing neurons in the microscopic image?

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In what part of the brain´s rabbit bdnf is located and include references please?

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