this could help you

http://pure.au.dk/portal/files/55280429/Novel_method_for_quantification_of_individual_free_fatty_acids_in_milk_using_an_in_solution_derivatisation_approach_and_gas_chromatography_mass_spectrometry.pdf

http://www.jove.com/visualize/abstract/21144530/direct-analysis-fatty-acid-profile-from-milk-thermochemolysis-gas

http://editorialbiogenesis.udea.edu.co/public/lipid.pdf

Hi...

  Following articles might interest you.

http://www.ncbi.nlm.nih.gov/pubmed/17296772

http://www.sciencedirect.com/science/article/pii/S092144881300028X

Regards:

Mahesh

I follow the protocoll for sheep milk reported in this work . For cow milk use 2 ml of milk instead of 1 ml.

regards

Fabio

we do fatty acid analysis on variety of food matrix using AOAC 996.06. This official method includes standard preparation, calculation, sample extraction details.

Dear Isidore, I'll send you the procedure for extraction milk lipid. following procedure was published in Serra et al. 2009 (Meat Sci. 2009 Feb;81(2):396-404)

Ammonia 25% (0.4 mL), ethyl alcohol 95% (1 mL) and hexane (5 mL) were added to 2 g of a raw milk sample. After being vortexed, the samples were centrifuged at 3,000 rpm and 2 °C. After phase’s separation, the upper layer was collected. The extraction was repeated a second time using ethyl alcohol 95% (1 mL) and hexane (5 mL); the samples were centrifuged at 3000 rpm and the upper layer was collected. After a third extraction using 5 mL of hexane, the samples were centrifuged at 3,000 rpm and the upper layer was collected. The extracted fat was dried at 35 °C by means of a rotary evaporator, weighed and finally dissolved in hexane.

Hi...

fat extraction methods are almost the same...but you have to decide before, if you going to determine free fatty acid or fatty acid methyl esters because the protocols are different.

Hi,

Thank you very much for your useful answers to my question.

Regards,